Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld Bis-Tris Propane, 500 g
A non-zwitterionic buffer that is useful in the pH 6.3-9.5 rangeSynonym: 1,3-Bis[tris(hydroxymethyl)methylamino]propane Appearance: SolidBoiling Point : 573.3 °CDensity : 1.326 g/cm3IUPAC_Name: 2,2'-(1,3-Propanediyldiimino)Bis[2-(Hydroxymethyl)-1,3-Propanediol]Melting Point: 164-165 °CPH Value: 6.3 - 9.5
Bioworld BES Buffer, 0.5M, pH 7.5, 250 mL
Product DescriptionBES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) buffer has a concentration of 0.5M and a pH value of 7.5. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Alkali Scientific SSC Buffer, 20X Solution, 4 L
SSC Buffer 20X Solution (4 L) is a large-format, ready-to-use buffer solution for high-throughput DNA hybridization experiments. This 20X concentration solution is essential for preparing a stable environment that supports the binding of nucleic acid probes to their complementary targets in hybridization protocols. SSC Buffer is commonly used in Southern and Northern blotting, as well as in situ hybridization and other DNA/RNA detection methods. The 4L volume is designed for laboratories conducting large-scale hybridization experiments, providing the necessary buffer for high-volume workflows while maintaining consistent ionic strength to ensure efficient and reliable hybridization.
Quality Biological Inc PBS (1X), pH 7.2, 4 Liters
PBS (phosphate buffered saline) is a balanced salt solution. Used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Also useful in immunological applications, such as Western Blotting.
Endotoxin and Mycoplasma tested
Without Calcium and Magnesium
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
pH: 7.1 -7.3
Osmolality: 300 ± 5% mOsm/Kg H2O
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Complement Technology Inc GVBE (GVBo with EDTA) 250ml
GVBE is an isotonic buffer used in complement assays to prevent complement activation or to stop ongoing complement activation when added at the end of an incubation
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659759.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 100 G
HEPES, Sodium Salt is a biochemical reagent available in a 100g package, used to maintain a stable pH environment in laboratory experiments. It is a highly effective buffer, particularly in biological systems, with an optimal pH range of 6.8–8.2. This salt form of HEPES is commonly used in cell culture, protein assays, and other biochemical processes where precise pH control is critical. It is non-toxic to cells, ensuring minimal interference with experimental outcomes. With its stable buffering capacity and versatility in a range of applications, HEPES sodium salt is a staple reagent in research and industrial laboratories.
![Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661289.jpg-250.jpg)
Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 500 G
TAPS, or Tris(hydroxymethyl) aminopropanesulfonic acid, is available in a high-purity free acid form for laboratory use. The 500g size is ideal for medium-scale buffer preparation, particularly in research and diagnostics. TAPS is widely used in biochemical, molecular biology, and clinical research applications to maintain a stable pH during experiments. The 500g quantity allows for frequent preparation of buffers without the need for constant restocking, providing researchers with a reliable, long-term supply. TAPS is critical for pH-sensitive reactions, including protein assays, electrophoresis, and enzyme studies, ensuring accuracy and consistency in experimental conditions.
Alkali Scientific Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 400mL, 20/Case
Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 400mL, 20/Case. This product is formulated for neutralizing disinfectants and enriching microbiological media. The sterile solution is ready-to-use and helps enhance the accuracy of lab tests by eliminating disinfectants in sample preparations. Suitable for use in microbiological analysis.
Research Products International Corp TES, Sodium Salt, 1 KG
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 100G
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 100G
Research Products International Corp Triton Lysis Buffer Solution, 500 ML
1X buffer used to lyse cells under non-denaturing conditions.
1X Formulation:
NaCI: 150 mM
TritonX-100: 1%
EDTA: 5 mM
GenDEPOT TBST(10X), pH 7.4 | 1L
TBST(10X) is a space-saving stock solution that is ideal for preparing TBS-Tween (TBS-T) wash buffers or blocking buffer diluent used for applications such as ELISA, Western blotting and other immunoassays. The 10X TBST (Tris-buffered saline with Tween™ 20 detergent) makes 25 mM Tris, 0.14M NaCl, 0.1% Tween-20, pH 7.4, when diluted to 1X with water.
Boston Bioproducts Inc Bicine Buffer (0.5 M, pH 8.5) - 250ml
Bicine Buffer (0 5 M pH 8 5) - 250ml (For Research Use and Further Manufacturing Only)
